Affiliation:
1. Anhui Engineering Research Center for Eco-Agriculture of Traditional Chinese Medicine, West Anhui University, Lu’an 237012, China
2. State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei 230036, China
Abstract
Polygonatum cyrtonema Hua, a source of Polygonati rhizoma, possesses multiple health-promoting properties and is widely used in food and medicine. This study aimed to elucidate the lifespan promotion abilities and potential mechanism of action of P. cyrtonema Hua polysaccharides (PCHPs) on Caenorhabditis elegans. The results revealed that PCHP had a molecular weight distribution of 8.77×103−1.84 × 106 Da and comprised Man, GalA, Glu, Gal, Xyl, and Ara in a molar ratio of 13.8 : 3.5 : 22.7 : 2.7 : 1:1.3. PCHP (5.0 mg/mL) markedly increased the lifespan of C. elegans by 15.78% (
< 0.001), improved motility, and increased the resistance to heat stress, UV irradiation, and oxidative stress. PCHP also promoted healthy aging by attenuating the accumulation of reactive oxygen species (ROS) and lipofuscin. Furthermore, PCHP upregulated the expression of oxidative stress- and aging-related genes, promoted the migration of DAF-16 into the nucleus, and exerted no effect on the lifespan of daf-2, daf-16, age-1, skn-1, and sir-2.1 mutants. These results indicate that the transcription factors DAF-16, SIR-2.1, and SKN-1 are required for PCHP-mediated lifespan extension in C. elegans. In conclusion, PCHP has the potential to attenuate the effects of aging, which can lay the foundation for aging treatment research with P. cyrtonema.
Subject
Cell Biology,Pharmacology,Food Science,Biophysics
Reference52 articles.
1. Ethnobotanical review of food uses of Polygonatum (Convallariaceae) in China
2. Advances in studies on chemical constituents of three medicinal plants from Polygonatum mill and their pharmacological activities;H. Chen;Chinese Traditional and Herbal Drugs,2015
3. Research progress on pharmacological effects of Polygonati Rhizoma;W. Zhao;Chinese Traditional and Herbal Drugs,2018
4. Analysis of chemical constituents from Polygonatum cyrtonema after “Nine-Steam-Nine-Bask” processing
5. Steroidal Saponins from Polygonatum cyrtonema
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