A Cytogenetic Study on the Efficacy of Chyawanprash Awaleha as an Antioxidant in Oral Premalignant Cancer

Abstract

Background. Chyawanprash awaleha (Cp) is an Ayurvedic rasayana formulation and is used as a genoprotective agent. Objective. The present cytogenetic study has been done to investigate the efficacy of Cp against betel quid chewers suffering from oral precancerous lesions through satellite association (SA) assay. Materials and Methods. The frequency of SA was analyzed in 21 betel quid chewing oral precancerous lesions patients and then they were divided into 2 groups. Group 1 consisted of 15 patients, advised to quit betel quid chewing and fed with 20 gms of Cp, twice a day for three months. Group 2 consisted of 6 patients, who refused Cp feed but accepted to quit betel quid chewing. At the end of three months, both groups were assessed cytogenetically. Results. The frequency of SA was statistically significant in both groups, but an elevated mean difference was observed more in Group 1 than in Group 2. Conclusion. The study indicates that betel quid cessation reduces the effect of DNA damage in oral precancerous lesions. But the increased mean difference in SA in Group 1 compared to Group 2 clearly indicates that Cp can further minimize the genotoxic effect caused by mutagenic agents present in betel quid.