A New Mn(II) Complex: Magnetic Property and Application Values in Foot and Ankle Arthritis

Abstract

The solvothermal reactions of C3-symmerical 4,4 ${}^{\prime }$ ,4 ${}^{\prime }$ -nitrilotribenzoic acid and MnCl2 afforded a novel Mn(II) compound, and its formula is [Mn3(TCA)2(e-urea)2(DMA)2]n·n(urea)·n(DMA) (1, H3TCA=4,4 ${}^{\prime }$ ,4 ${}^{\prime }$ -nitrilotribenzoic acid, e-urea=2-imidazaolidone, DMA=N,N ${}^{\prime }$ -dimethylacetamide). Magnetic property investigation of 1 indicates weak antiferromagnetic mutual effects exist between neighboring Mn(II) ions. Serial biological tests were adopted to discover new compound activity. Firstly, the enzyme-linked immunosorbent assay (ELISA) was conducted to measure the content of inflammatory cytokines released into the synovial fluid. In addition, we also studied adenosine 5 ${}^{\prime }$ -monophosphate- (AMP-) activated protein kinase (AMPK) inflammatory signaling pathway activation through real-time reverse transcription-polymerase chain reaction (RT-PCR).