Affiliation:
1. Department of Basic Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
2. Graduate of Faculty of Veterinary Sciences, Sanandaj Branch, Islamic Azad University, Sanandaj, Iran
3. Department of Physiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran
Abstract
Occupational and environmental exposure to heavy metals such as arsenic (As) and lead (Pb) by inducing oxidative damage may impair male fertility. However, there is a new view that shows that the nano form of vitamins such as vitamin C, which have antioxidant activity, can be effective in improving this disorder. Therefore, this study aimed to evaluate the effect of NVC (NVC) on reproductive toxicity caused by the combination of Pb and As on testicular histology, sperm morphology, oxidative stress parameters, and hormonal changes in male rats. In this experimental study, forty-two male Wistar rats were randomly divided into six groups: control, NVC (200 mg/kg), As (50 ppm sodium arsenate), Pb (500 ppm Pb acetate), As + NVC, and Pb + NVC. FSH, LH, and testosterone levels were measured in serum. The activity of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), carbonyl protein, malondialdehyde (MDA), and total antioxidant capacity (TAC) was measured in testis. Histological examination and sperm parameters were also evaluated. FSH, LH, and testosterone levels and sperm parameters significantly decreased, and levels of protein carbonyl, MDA, and DNA fragmentation increased in the As and Pb groups, while treatment with NVC could improve them. Histological evaluation and sperm parameters in As and Pb groups showed damage in the process of spermatogenesis and sperm parameters. The treatment with NVC could significantly improve these parameters. The activity of GPx, SOD, and CAT in testis decreased in As and Pb groups, while treatment with NVC could enhance them. It can be concluded that NVC by inhibiting oxidative damage and improving serum level of testosterone, LH, and FSH could overcome As- and Pb-induced reproductive dysfunction.
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