MiR-526b-3p Inhibits the Resistance of Glioma Cells to Adriamycin by Targeting MAPRE1

Author:

Qiu Lei1ORCID,Xing Shuyuan2ORCID,Lang Fuxin3ORCID,Bao Qing45ORCID

Affiliation:

1. Department of Neurosurgery, Zibo Central Hospital, Zibo, Shandong Province 255000, China

2. Department of Neurosurgery, Zouping Hospital of Traditional Chinese Medicine, Zouping City, Shandong Province 256200, China

3. Department of Neurosurgery, Huantai People’s Hospital, Zibo, Shandong Province 256400, China

4. Department of Neurosurgery, Wujin Hospital Affiliated with Jiangsu University, Changzhou City, Jiangsu Province 213100, China

5. Department of Neurosurgery, The Wujin Clinical College of Xuzhou Medical University, Changzhou City, Jiangsu Province 213100, China

Abstract

Background. Cell resistance is the main reason for the high mortality in glioma. Adriamycin (ADR) is a treatment drug for glioma and often leads to chemoresistance. Previous studies have confirmed that the abnormal expression of microRNA (miRNA) affects the resistance of glioma cells. Methods. RT-qPCR and western blot were conducted for detecting miR-526b-3p levels and related protein expressions. CCK8 assay, colony formation, flow cytometry, and Transwell were adopted to assess cell viability, proliferation, apoptosis, and metastasis. Moreover, downstream targets of miR-526b-3p were identified through a dual-luciferase reporter and RNA pull-down analysis. Results. Nevertheless, miR-526b-3p functions on glioma cell resistance to ADR are not well characterized. This study demonstrated that miR-526b-3p levels were decreased within glioma cells and further decreased within ADR-resistant glioma cells. Then, miR-526b-3p overexpression repressed glioma cell proliferation and invasion while inducing cell apoptosis. Overexpression of miR-526b-3p within ADR-resistant glioma cells obtained similar results, which suggested miR-526b-3p suppressed glioma cell resistance to ADR. Mechanistically, miR-526b-3p targeted MAPKE1 and negatively regulated MAPKE1 expressions. Restoration of MAPKE1 levels reversed miR-526b-3p effects on the glioma process and resistance to ADR. Conclusion. These results suggest that miR-526b-3p acts as a diagnostic marker in glioma development and therapeutic target of the glioma resistance to ADR.

Publisher

Hindawi Limited

Subject

Oncology

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