Proteomic Profiling of Thyroid Papillary Carcinoma-Reference-Cited by-同舟云学术

Proteomic Profiling of Thyroid Papillary Carcinoma

Published:2012 Issue: Volume:2012 Page:1-7
ISSN:2090-8067
Container-title:Journal of Thyroid Research
language:en
Short-container-title:Journal of Thyroid Research

Author:

Ban Yoshiyuki¹,Yamamoto Gou²,Takada Michiya¹,Hayashi Shigeo²,Ban Yoshio³,Shimizu Kazuo⁴,Akasu Haruki⁴,Igarashi Takehito⁴,Bando Yasuhiko⁵,Tachikawa Tetsuhiko²⁶,Hirano Tsutomu¹

Affiliation:

1. Division of Diabetes, Metabolism and Endocrinology, Department of Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8666, Japan

2. Department of Oral Pathology and Diagnosis, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan

3. Ban Thyroid Clinic, 2-11-16 Jiyugaoka, Megro-ku, Tokyo 152-0035, Japan

4. Division of Endocrine Surgery, Department of Surgery, Nippon Medical School, 1-1-5 Sendagi, Bunkyo-ku, Tokyo 113-8602, Japan

5. Biosys Technologies, Inc., 2-13-18 Nakane, Meguro-ku, Tokyo 152-0031, Japan

6. Comprehensive Research Center of Oral Cancer, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555, Japan

Abstract

Papillary thyroid carcinoma (PTC) is the most common endocrine malignancy. We performed shotgun liquid chromatography (LC)/tandem mass spectrometry (MS/MS) analysis on pooled protein extracts from patients with PTC and compared the results with those from normal thyroid tissue validated by real-time (RT) PCR and immunohistochemistry (IHC). We detected 524 types of protein in PTC and 432 in normal thyroid gland. Among these proteins, 145 were specific to PTC and 53 were specific to normal thyroid gland. We have also identified two important new markers, nephronectin (NPNT) and malectin (MLEC). Reproducibility was confirmed with several known markers, but the one of two new candidate markers such as MLEC did not show large variations in expression levels. Furthermore, IHC confirmed the overexpression of both those markers in PTCs compared with normal surrounding tissues. Our protein data suggest that NPNT and MLEC could be a characteristic marker for PTC.

Funder

Showa University

Publisher

Hindawi Limited

Subject

Endocrinology, Diabetes and Metabolism

Link

http://downloads.hindawi.com/journals/jtr/2012/815079.pdf

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