The Role of Fecal Fusobacterium nucleatum and pks+ Escherichia coli as Early Diagnostic Markers of Colorectal Cancer

Author:

Liu Kaixi1,Yang Xinran2,Zeng Mi3,Yuan Yumeng3,Sun Jianhong4,He Ping3,Sun Jiayu3,Xie Qingdong3,Chang Xiaolan3,Zhang Suwei1,Chen Xiang5,Cai Leshan2,Xie Yanxuan2,Jiao Xiaoyang3ORCID

Affiliation:

1. Departments of Clinical Laboratory, Shantou Central Hospital, Shantou, China

2. Departments of Clinical Laboratory, The First Affiliated Hospital of Shantou University Medical College, China

3. Medical College of Shantou University, Shantou, China

4. Departments of Clinical Pathology, Shantou Central Hospital, Shantou, China

5. Departments of Health Care Center, The First Affiliated Hospital of Shantou University Medical College, China

Abstract

Background. Accurate analysis of intestinal microbiota will facilitate establishment of an evaluating system for assessing colorectal cancer (CRC) risk and prognosis. This study evaluates the potential role of Fusobacterium nucleatum (F. nucleatum) and Escherichia coli with a pks gene (pks+ E. coli) in early CRC diagnosis. Methods. We recruited 139 patients, including CRC ( n = 60 ), colorectal adenomatous polyposis (CAP) ( n = 37 ), and healthy individuals ( n = 42 ) based on their colonoscopy examinations. We collected stool and serum samples from the participants and measured the relative abundance of F. nucleatum and pks+ E. coli in fecal samples by quantitative PCR. Receiver operating characteristic curve (ROC) analyses were used to analyze the diagnostic value of single or combined biomarkers. Results. Fecal F. nucleatum and pks+ E. coli levels were higher in the CRC group in either the CAP group or healthy controls ( P = 0.02 ; 0.01). There was no statistical difference in the distribution of F. nucleatum and pks+ E. coli in patients with different tumor sites ( P > 0.05 ). The combination of F. nucleatum+pks+ E. coli+CEA+CA19-9+FOBT was chosen as the optimal panel in differentiating both CRC and CAP from the controls. The combination of F. nucleatum, pks+ E. coli, and FOBT improved diagnostic efficiency. However, there was difficulty in differentiating CRC from CAP. Conclusion. Our results suggested that combining bacterial markers with conventional tumor markers improves the diagnostic efficiency for noninvasive diagnosis of CRC.

Funder

2020 Li Ka Shing Foundation Cross-Disciplinary Research Grant

Publisher

Hindawi Limited

Subject

Biochemistry (medical),Clinical Biochemistry,Genetics,Molecular Biology,General Medicine

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