Effects of Juniperus phoenicea Hydroalcoholic Extract on Inflammatory Mediators and Oxidative Stress Markers in Carrageenan-Induced Paw Oedema in Mice

Author:

Zouari Bouassida Karama1ORCID,Makni Samar1,Tounsi Amina1,Jlaiel Lobna2,Trigui Mohamed1ORCID,Tounsi Slim1ORCID

Affiliation:

1. Biopesticides Laboratory (LPIP), Center of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia

2. Analysis Department, Center of Biotechnology of Sfax, University of Sfax, P.O. Box 1177, 3018 Sfax, Tunisia

Abstract

Juniperus phoenicea (J. phoenicea) is a wild tree belonging to the Cupressaceae family, commonly used for the treatment of several disorders. This study aimed to evaluate the potential protective effects of J. phoenicea hydroethanolic extract (EtOH-H2OE) against oxidation, acute inflammation, and pain in mice models. For the purpose, chemical compounds of J. phoenicea EtOH-H2OE were also analyzed by GC-MS. The J. phoenicea EtOH-H2OE showed a potent antioxidant activity in vitro, thanks to its richness in phenolic and flavonoid compounds. Mice treated with EtOH-H2OE (100 mg/kg BW) showed reduced paw oedema formation and decreased malondialdehyde (MDA) content. The evaluation of antioxidant enzyme activities in paw oedema tissue after five hours of carrageenan induction showed a significant increase (P<0.05). Inflammatory biomarkers explorations of J. phoenicea EtOH-H2OE-treated mice showed a restoration of the studied parameters to near-normal values. Furthermore, EtOH-H2OE of J. phoenicea produced a significant reduction of the number of abdominal writhes (P<0.05) in a dose-dependent way. Phytochemical analysis of the J. phoenicea EtOH-H2OE by GC-MS showed the presence of hexadecanoic and stearic acids known as anti-inflammatory and analgesic compounds. Our investigation provided evidence that J. phoenicea EtOH-H2OE can effectively reduce the inflammation and pain in mice models.

Funder

Tunisian Ministry of Higher Education and Scientific Research

Publisher

Hindawi Limited

Subject

General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

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