Antilisterial Effect ofRosa damascenaandNymphaea albainMus musculus

Abstract

The present study was proposed to investigate the toxicological and prophylactic potential of ethanolic extracts ofRosa damascenaandNymphaea albaand their mixture in albino mice. For toxicity study, three different doses of plant extracts were orally administrated to three groups of mice for 14 successive days. Blood biochemistry and histological examinations of liver and kidney revealed that these extracts had no harmful effects up to 1000 mg/kg. To determine the prophylactic effects ofRosa damascena,Nymphaea alba, and their mixture, an infection model ofListeria monocytogeneswas established in a pilot study. Establishment of infection was confirmed by changes in haematological parameters and reisolation ofListeria monocytogenesfrom different tissues. Results showed that these extracts alone or in combination could restrict the growth ofListeria monocytogenesin different organs. Neutrophils were high in positive control group but remained in normal range in all treated groups.Listeria monocytogeneswas recovered in low numbers from animals treated with extract of single plant but was negligible in group treated with mixture of extract of plants. Platelets count was increased in treated groups as compared to control. Results confirmed that these extracts are potent source of antimicrobial compounds and that they have synergistic effect in combined form.