Bioactivity of Root Endophytic Freshwater HyphomycetesAnguillospora longissima(Sacc. & Syd.) Ingold

Abstract

Anguillospora longissima, isolated from root as endophytic freshwater hyphomycetes, was evaluated for its bioactivity (antibacterial potential) against five bacterial strains, namely, Gram-positive (Bacillus subtilisMTCC 121) and Gram-negative (Agrobacterium tumefaciensMTCC 609,Escherichia coliMTCC 40,Erwinia chrysanthemum,andXanthomonas pseudomonas). Antimicrobial activity was assessed by measuring the zone of inhibition with preliminary and secondary antimicrobial assays. The applied fungus was found significant for all tested bacterial strains as showen by their zone of inhibition. In preliminary antimicrobial assay, maximum zone of inhibition was recorded against Gram-negative human pathogenic bacterial strainEscherichia coli(23 mm) followed byErwinia chrysanthemi(22 mm),Agrobacterium tumefaciens(21 mm), andXanthomonas phaseoli(21 mm), while minimum zone of inhibition was observed againstBacillus subtilis(20 mm). In secondary antimicrobial assay, the maximum zone of inhibition was recorded againstErwinia chrysanthemi(11 mm) followed byAgrobacterium tumefaciens(10 mm),Xanthomonas phaseoli(10 mm), andBacillus subtilis(9 mm) and minimum inhibition was found againstEscherichia coli(8 mm).