A Ratiometric Fluorescence Imaging System for Surgical Guidance-Reference-Cited by-同舟云学术

A Ratiometric Fluorescence Imaging System for Surgical Guidance

Published:2008-10-16 Issue: Volume:2008 Page:1-10
ISSN:1687-6393
Container-title:Advances in Optical Technologies
language:en
Short-container-title:Advances in Optical Technologies

Author:

Moriyama Eduardo H.¹²,Kim Anthony¹,Bogaards Arjen¹,Lilge Lothar¹,Wilson Brian C.¹

Affiliation:

1. Division of Biophysics and Bioimaging, Ontario Cancer Institute, University Health Network, 610 University Avenue 7-420, Toronto, ON, Canada M5G 2M9

2. Department of Physics, Ryerson University, 350 Victoria Street, Toronto, ON, Canada M5B 2K3

Abstract

A 3-chip CCD imaging system has been developed for quantitative in vivo fluorescence imaging. This incorporates a ratiometric algorithm to correct for the effects of tissue optical absorption and scattering, imaging “geometry” and tissue autofluorescence background. The performance was characterized, and the algorithm was validated in tissue-simulating optical phantoms for quantitative measurement of the fluorescent molecule protoporphyrin IX (PpIX). The technical feasibility to use this system for fluorescence-guided surgical resection of malignant brain tumor tissue was assessed in an animal model in which PpIX was induced exogenously in the tumor cells by systemic administration of aminolevulinic acid (ALA).

Funder

National Institutes of Health

Publisher

Hindawi Limited

Subject

Atomic and Molecular Physics, and Optics,Electronic, Optical and Magnetic Materials

Link

http://downloads.hindawi.com/archive/2008/532368.pdf

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