miRNA Expression and Interaction with Genes Involved in Susceptibility to Pristane-Induced Arthritis

Author:

Fernandes Jussara Gonçalves1,Borrego Andrea1,Jensen José Ricardo1ORCID,Cabrera Wafa Hanna Koury1ORCID,Correa Mara Adriana1,Starobinas Nancy1ORCID,Ribeiro Orlando Garcia1ORCID,Ibañez Olga Martinez1ORCID,De Franco Marcelo12ORCID

Affiliation:

1. Laboratório de Imunogenética, Instituto Butantan, São Paulo 05503000, Brazil

2. Seção de Diagnóstico, Instituto Pasteur, São Paulo 01311000, Brazil

Abstract

Pristane-induced arthritis (PIA) in mice is an experimental model that resembles human rheumatoid arthritis, a chronic autoimmune disease that affects joints and is characterized by synovial inflammation and articular cartilage and bone destruction. AIRmax and AIRmin mouse lines differ in their susceptibility to PIA, and linkage analysis in this model mapped arthritis severity QTLs in chromosomes 5 and 8. miRNAs are a class of small RNA molecules that have been extensively studied in the development of arthritis. We analyzed miRNA and gene expression profiles in peritoneal cells of AIRmax and AIRmin lines, in order to evaluate the genetic architecture in this model. Susceptible AIRmax mice showed higher gene (2025 vs 1043) and miRNA (240 vs 59) modulation than resistant AIRmin mice at the onset of disease symptoms. miR-132-3p/212-3p, miR-106-5p, miR-27b-3p, and miR-25-3p were among the miRNAs with the highest expression in susceptible animals, showing a negative correlation with the expression of predicted target genes (Il10, Cd69, and Sp1r1). Our study showed that global gene and miRNA expression profiles in peritoneal cells of susceptible AIRmax and resistant AIRmin lines during pristane-induced arthritis are distinct, evidencing interesting targets for further validation.

Funder

Conselho Nacional de Desenvolvimento Científico e Tecnológico

Publisher

Hindawi Limited

Subject

Immunology,General Medicine,Immunology and Allergy

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