Optimization and Characterization of Silver Nanoparticle by Endophytic FungiPenicilliumsp. Isolated fromCurcuma longa(Turmeric) and Application Studies against MDRE. coliandS. aureus

Abstract

Development of ecofriendly and reliable processes for the synthesis of nanoparticles has attracted considerable interest in nanotechnology because of its tremendous impetus in modulating metals into nanosize to their potential use for human benefits. In this study an endophytic fungus,Penicilliumsp., isolated from healthy leaves ofCurcuma longa(turmeric) was subjected to extracellular biosynthesis of silver nanoparticles (AgNps) and their activity against MDRE. coliandS. aureus. The biosynthesized AgNps optimization was studied and characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), and transmission electron microscopy (TEM). Then produced AgNps were tested against MDRE. coliandS. aureus. The endophytic fungusPenicilliumsp. from healthy leaves ofC. longa(turmeric) was found to be a good producer of AgNps. Parametric optimization showed maximum absorbance of 420–425 nm at pH-7, 25°C with 1 mM AgNO3concentration and 15–20 g of wet biomass. Further TEM revealed the formation of spherical, well-dispersed nanoparticles with size ranging between 25 and 30 nm and FTIR shows the bands at 1644 and 1538 cm−1corresponding to the binding vibrations of amide I and II bands of proteins, respectively. Antibacterial activity against MDRE. coliandS. aureusshowed good results showing maximum zone of inhibition of 17 mm and 16 mm, respectively, at 80 µL of AgNps.