Neuroprotective Role of a Novel Copper Chelator againstAβ42Induced Neurotoxicity

Abstract

Alzheimer's disease (AD) is a progressive neurodegenerative disease and associated with the extracellular deposits of amyloid-βpeptide in hippocampus region. Metal ions like Cu, Fe and Zn are known to associate with the amyloid beta (Aβ) at high concentration and interaction of these ions with soluble and aggregated forms of Aβpeptide help in development of AD. Here we showed Cu mediated neurotoxicity in the eye tissues of transgenicDrosophilaexpressing human amyloidβand its rescue through a novel Cu chelator. In this context, we have synthesised and characterized the compound L 2,6-Pyridinedicarboxylic acid, 2,6-bis[2-[(4-carboxyphenyl) methylene] hydrazide] by Mass spectra (MS) and Elemental analysis (EA). The Cu chelation potential of the compound L is testedin vivoinDrosophila. Oral administration of Copper to the transgenic larvae resulted in severe degeneration in eye tissues, which was rescued by the supplementation of compound L. The levels of anti-oxidant markers like SOD and MDA were measured in compound L treated flies and found a significant rescue (P<0.001). Further rescue of the eye degeneration phenotypes as revealed by SEM affirm the role of copper in Aβtoxicity. Hence, use of compound L, an amidoamine derivative, could be a possible therapeutic measure for Aβinduced neurotoxicity.