Combined Overlap Extension PCR Method for Improved Site Directed Mutagenesis
Author:
Affiliation:
1. Department of Molecular Biology, Faculty of Resource Science and Technology, Universiti Malaysia Sarawak, 94300 Kota Samarahan, Sarawak, Malaysia
Abstract
Funder
Ministry of Higher Education Malaysia
Publisher
Hindawi Limited
Subject
General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine
Link
http://downloads.hindawi.com/journals/bmri/2016/8041532.pdf
Reference16 articles.
1. A rapid and efficient method for site-directed mutagenesis using one- step overlap extension PCR
2. Asymmetric overlap extension PCR method bypassing intermediate purification and the amplification of wild-type template in site-directed mutagenesis
3. Improvement of megaprimer method for site-directed mutagenesis and its application to phytase
4. Generation of various amino acids mutants in the trpR gene of Escherichia coli by site-directed mutagenesis
5. Improved PCR-based gene synthesis method and its application to the Citrobacter freundii phytase gene codon modification
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