In VitroVitamin K3Effect on Conjunctival Fibroblast Migration and Proliferation

Abstract

Purpose. To evaluate the dose effect of vitamin K3on wound healing mechanisms.Methods. Conjunctival fibroblasts were incubated for 24 hours. An artificial wound was made and the cells were incubated with fresh medium plus doses of vitamin K3to be tested. Wound repair was monitored at 0, 18, 24, and 48 hours. Proliferation was measured in actively dividing cells by [3H]thymidine uptake. Six different groups were tested: group 1/no drugs added, group 2/ethanol 0.1%, group 3/vitamin K31 mg/L, group 4/vitamin K32 mg/L, group 5/vitamin K34 mg/L, and group 6/vitamin K36 mg/L. Each experiment was carried out in triplicate and 4 times.Results. There were no differences among groups at the initial time.In vitrowound repair was slower in groups 4, 5, and 6. There were no differences between control and ethanol groups and between control and vitamin K31 mg/L groups. Fibroblast mitogenic activity was statistically decreased in all vitamin K groups; statistical differences were found among vitamin K31 mg/mL and higher doses too. In groups 5 and 6, cellular toxicity was presented.Conclusions. Vitamin K3is able to inhibit fibroblast proliferation. Vitamin K32 mg/L or higher doses inhibit wound healing repair, exhibiting cellular toxicity at 4 and 6 mg/L.