The multiplex PCR assay detection of Staphylococcus sciuri antibiotic resistance, mecA gene, and the inhibitory effect of root exudate of Nigella sativa (black seeds) treated with magnetized water

Abstract

121 bacterial samples isolated from wounds from both sexes and all age groups were collected from Salahadin General Hospital, Salahadin provenance, Iraq. Only 8 Staphylococcus sciuri (S. sciuri) isolates were identified. The bacterial isolation showed the highest sensitivity to Amoxicillin/Clavulanic acid, Cefotaxime, Methicillin, Streptomycin, and Vancomycin and resistance to all other antibiotics. The root exudates of black seeds were used for 10 and 20 days for both treatments with and without magnetized water, and the exudates were superior when using magnetized water for 20 days. Antibiotic resistance and the mecA gene were investigated, and a multiplex PCR assay was used to detect the mecA gene in S. sciuri. Optimized conditions were used to amplify mecA fragments that encode methicillin resistance.