Nanoparticle diffusion in spontaneously expectorated sputum as a biophysical tool to probe disease severity in COPD

Author:

Chisholm Jane F.,Shenoy Siddharth K.,Shade Julie K.,Kim Victor,Putcha Nirupama,Carson Kathryn A.ORCID,Wise RobertORCID,Hansel Nadia N.,Hanes Justin S.,Suk Jung Soo,Neptune Enid

Abstract

Perturbations in airway mucus properties contribute to lung function decline in patients with chronic obstructive pulmonary disease (COPD). While alterations in bulk mucus rheology have been widely explored, microscopic mucus properties that directly impact on the dynamics of microorganisms and immune cells in the COPD lungs are yet to be investigated.We hypothesised that a tightened mesh structure of spontaneously expectorated mucus (i.e. sputum) would contribute to increased COPD disease severity. Here, we investigated whether the mesh size of COPD sputum, quantified by muco-inert nanoparticle (MIP) diffusion, correlated with sputum composition and lung function measurements.The microstructure of COPD sputum was assessed based on the mean squared displacement (MSD) of variously sized MIPs measured by multiple particle tracking. MSD values were correlated with sputum composition and spirometry. In total, 33 samples collected from COPD or non-COPD individuals were analysed.We found that 100 nm MIPs differentiated microstructural features of COPD sputum. The mobility of MIPs was more hindered in sputum samples from patients with severe COPD, suggesting a tighter mucus mesh size. Specifically, MSD values inversely correlated with lung function.These findings suggest that sputum microstructure may serve as a novel risk factor for COPD progression and severity.

Funder

National Institutes of Health

Publisher

European Respiratory Society (ERS)

Subject

Pulmonary and Respiratory Medicine

Reference56 articles.

1. Mucus hypersecretion in chronic obstructive pulmonary disease;Rogers;Novartis Found Symp,2001

2. Revisited role for mucus hypersecretion in the pathogenesis of COPD

3. CFTR, Mucins, and Mucus Obstruction in Cystic Fibrosis

4. Mucus hypersecretion in asthma: causes and effects

5. Deaths: preliminary data for 2011;Hoyert;Natl Vital Stat Rep,2012

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