Effect of Feeding Rat on the Biochemical Profile of Blood and Liver Morphology

Abstract

The nature of nutrition modifies the metabolism of substances and ensures either normal functioning or contributes to metabolic disorders and the development of pathologies. A study was made of the effect of different feeding diets (lipid, carbohydrate and protein) on the blood biochemical profile of adult sexually mature rats. The work was performed on 28 white laboratory male rats weighing 180–250 g, which were 4 groups: the first (1) group (n=7) was fed exclusively with lipids (fat tail fat), the second (2, n=7) — carbohydrates (sugar), the third (3, n=7) — proteins, and the fourth (4, n=7) — standard food. The levels of glucose, cholesterol, total protein and albumin were determined by the colorimetric method. It was revealed that when taking lipid food in the blood, levels of cholesterol, glucose, total protein and albumin increased; when consuming carbohydrate feed — the content of glucose and lipids; when consuming a protein diet — total protein, albumin and lipids. The concentration of cholesterol increased with the use of all types of diet, especially significantly with lipid. Thus, glucose concentration increased with carbohydrate and lipid diets; total protein — with protein; glucose equally — with lipid and carbohydrate. The highest concentration of albumin is observed with a lipid diet, the lowest — with a carbohydrate.