Genetic Diversity in the Vigna Savi Collection and Characteristics Using Molecular Markers

Abstract

Genetic diversity was assessed by biomorphological characters and ISSR markers in a collection of 30 accessions belonging to the genus Vigna Savi. In the studied collection, great variability in biomorphological characteristics was observed; genotypes with superior indicators were selected. The highest yield of K-259 (108 g) and Vigna unguiculata subsp. sesquipedalis (L.) Verdc. (250 g). Among Vignaaccessions, the height to the first pod was 12.5-47.0 cm, which indicates that the vast majority of the studied genotypes are suitable for mechanized harvesting. For 30 samples with ISSR markers, a total of 22 alleles were amplified and genotypes with unique alleles in the collection were identified. The average genetic diversity index of the collection was very high — 0.73 units, indicating high genetic diversity between V. radiata (L.) R. Wilczek and V. unguiculata (L.) Walp. accessions. An average of 73% polymorphism was identified using ISSR markers. The results obtained make it possible to select genetically different samples with the best economic indicators and use them as parental forms to obtain recombinants of breeding value.