Human sperm parameter improvement associated with Ceratonia siliqua extract as a cryopreservation supplement after vitrification

Author:

Farazmand Tooba,Mansouri Fatemeh,Koohestanidehaghi Yeganeh,Shahandeh Erfan

Abstract

Objective: Given the destructive effects of oxidative stress on sperm structure, this study was conducted to investigate the antioxidant effects of different concentrations of <i>Ceratonia siliqua</i> plant extract on human sperm parameters after the freezing-thawing process.Methods: A total of 20 normozoospermic samples were frozen. Each sample was divided into two control groups (fresh and cryopreservation) and three cryopreservation experimental groups (containing <i>C. siliqua</i> extract at concentrations of 20, 30, and 40 μg/mL in the freezing extender). Motility, intracellular levels of reactive oxygen species (ROS), plasma membrane integrity (PMI), mitochondrial membrane potential (MMP), viability, and acrosome reaction parameters were evaluated.Results: Statistical analysis showed that the highest motility, viability, and PMI were associated with the 20 μg/mL concentration of <i>C. siliqua</i> extract. At all concentrations, intracellular ROS levels were significantly lower and the levels of MMP and the acrosome reaction were significantly higher than in the cryopreservation control group (<i>p</i>≤0.05).Conclusion: <i>C. siliqua</i> extract supplements at concentrations of 20, 30, and 40 μg/mL improved sperm motility, viability, PMI, MMP, intracellular ROS, and the acrosome reaction.

Publisher

The Korean Society for Reproductive Medicine

Subject

Reproductive Medicine

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3