Affiliation:
1. Department of Horticultural Science, College of Agriculture, Shiraz University, Shiraz, Iran
2. Nuclear Agriculture Research School, Nuclear Science and Technology Research Institute
(NSTRI), Karaj, Iran
3. Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King
Faisal University, Al-Ahsa 31982, Saudi Arabia
Abstract
In tissue culture, plants are genetically identical to native plants. Using
methods such as flow cytometry, cytogenetic analysis, and molecular markers such as
AFLP, ISSR, RAPD, RFLP, and SSR, we can detect the genetic uniformity of plants.
Among these techniques, flow cytometry (FCM) is a fast, easy, cost-effective, and
accurate method for screening the genetic stability of propagated plants. FCM involves
measuring the fluorescence light of cell nuclei with a flow cytometer after separation
and staining with a chemical with fluorescence properties related to DNA. There is a
computer with software for receiving, storing, further processing, and displaying result
information. The information is presented in an uncomplicated diagram. FCM is used
to determine the genome size and ploidy levels of plants produced In Vitro. FCM also
stimulates cell cycle function and replication rate in various plant organs and tissues. It
was used to study plant organs in greenhouse/field conditions and laboratory conditions
(anther culture, eggs, and protoplasts). Plant materials grown in tissue culture are
unstable due to somaclonal diversity, especially in their DNA content, and therefore,
the use of the FCM method is very effective.
Publisher
BENTHAM SCIENCE PUBLISHERS