The Production and Analysis of Biodegradable Polymers of Type of Medium-Chain-Length Polyhydroxyalkanoates (mcl-PHA) by Pseudomonas putida Strain for the Biomedical Engineering

Abstract

Background: Polyhydroxyalkanoates (PHAs) are bacteria-synthetized biopolymers under unbalanced growth conditions. These biopolymers are considered potential biomaterials for future applications for their biocompatibility and biodegradable features and potential biomaterials for future applications for their biocompatibility and biodegradable characteristics and their ability to be quickly produced and functionalize with strong mechanical resistance. This article is intended to perform microbial fermentation using Pseudomonas putida strain to show the amount of biopolymers of the type polyhydroxyalkanoates with medium-chain-length (mcl-PHA) obtained depending on the type and quantity of added precursors (glucose and fatty acids). Methods: It is important to understand the microbial interaction and mechanism involved in PHA biosynthetis.For these, several methods were used, such as: obtaining microbial biomass by using a Pseudomonas putida strain able of PHA-producing, analysis of biopolymer production by acetone extraction following the Soxhlet method, purification of biopolymer by methanol-ethanol treatment, followed by the estimation of biomass by spectrophotometric analysis and the measurement of the dry weight of cells and the quantification of the amount of biopolymer produced following the gas chromatographic method (GC). Results: The highest PHA yield was obtained using octanoic (17 mL in 2000 mL medium) and hexanoic acids (14 mL in 2000 mL medium) as precursors. Consequently, octanoic acid – octanoic acid, heptanoic acid – nonanoic acid, and octanoic acid - hexanoic acid were the mix of precursors that supported the amount of PHA obtained. Conclusion: Of the 4 types of structurally related substrate, the strain Pseudomonas putida ICCF 319 prefers the C8 sublayer for an elastomeric PHA's biosynthesis with a composition in which the C8 monomer predominates over C6 and C10.