Affiliation:
1. Department of Systems Biology and Engineering, Silesian University of Technology, 16 Akademicka Str., 44-100 Gliwice, Poland
2. Student’s Scientific Society of Biotechnology at the Biotechnology Centre, Silesian University of Technology, Krzywoustego 8, 44-100 Gliwice, Poland
Abstract
Background:
Changes in the cellular behavior depend on environmental and intracellular
interactions. Cancer treatments force the changes, first on the molecular level, but the main visible
changes are macroscopic. During radiotherapy, cancer cell’s adhesion, proliferation and migration
should be well monitored. In over 60% of diagnosed cancers cases, patients are given treatments with
different protocols of radiotherapy, which result in possible metastasis and acute whole body response
to toxic radiation.
Objective:
Effectiveness of the therapy used depends on the sensitivity/resistance of irradiated cancer
cells. Cellular mechanisms of cancer protection, such as the activation of DNA damage and repair
pathways, antioxidants production and oxidative stress suppression during treatments are not desirable.
Cancer cells monitoring require the development of novel techniques, and the best techniques are
non-invasive and long-term live observation methods, which are shown in this study.
Methods:
In cancers, invasive and metastatic phenotypes could be enhanced by stimulation of proliferation
rate, decreased adhesion with simultaneous increase of motility and migration potential. For such
reasons, the Ionizing Radiation (IR) stimulated proliferation; migration with lowered adhesiveness of
cancer Me45 and normal fibroblasts NHDF were studied. Using impedance measurements technique
for live cells, the adhesion of cells after IR exposition was assessed. Additionally proliferation and migration
potential, based on standard Wound Healing assay were evaluated by timelapse microscopic
observations.
Results:
We found simulative IR dose-ranges (0.2-2 Gy) for Me45 and NHDF cells, with higher proliferation
and adhesion rates. On the other hand, lethal impact of IR (10-12 Gy) on both the cell lines
was indicated.
Conclusion:
Over-confluence cell populations, characterized with high crowd and contact inhibition
could modulate invasiveness of individual cells, convert them to display migration phenotype and advance
motility, especially after radiotherapy treatments.
Funder
Silesian University of Technology
Polish National Science Center
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmaceutical Science,Biotechnology
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