Affiliation:
1. School of Biotechnology, Maduai Kamaraj University, Madurai 625021, India
Abstract
Background:
Foot and mouth disease (FMD) is caused by a virus of the genus Aphthovirus,
family Picornaviridae which includes several members of medical importance, Multiple subtypes or antigenic
variants within each serotype, which make the vaccine from one serotype does not confer protection
against the other serotype.
Methods:
Green synthesized silver nanoparticles were functionalized with FMDV antigen /antibody.
The functionalized silver nanoparticles were characterized by UV -Visible spectrophotometer, Fluorescence
Spectrophotometer etc. Immunomodulation study, efficacy and toxicity tests on the final product
were carried out.
Results:
The protein profile after immunoprecipitation with AntiFMD antibody analysed on a 12.5%
SDS-PAGE which corresponded to the viral proteins. The western blot analysis confirmed the same pattern.
When the infected mice were treated with functionalised silver nanoparticles, all mice were
recovered from the disease within 12 hrs. The field trial of these nanoformulations showed 100%
recovery of the animals with minimum neutralizing antibody without any other physiological problems.
Conclusion:
Surface modification of silver nanoparticles can create multifunctional materials with potential
applications. Nanoformulations developed by functionalizing whole FMD viral protein /antibody
with that of silver nanoparticles, elicite an optimal immuno-protective response and as diagnostic agent
against foot and mouth disease causing virus The easy method of preparation of nanoparticle, the flexibility
of functionalization techniques, long shelf life without cold chain protection and minimum single
low dosage reveals the feasibility of this nanoformulation applications ranging from prophylactic vaccines,
diagnostics, therapy for all infections leading to autoimmune diseases.
Publisher
Bentham Science Publishers Ltd.
Subject
General Engineering,General Materials Science
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