Marine Actinomycetes Derived Pyrrolo Compounds Mediated Green Synthesis of AgO and Ag2O3 Nanoparticles and its Antidermatophytic Activity

Abstract

Background: Antimicrobial potential of silver nanoparticles synthesised by using various biological sources was already been reported by many researchers. The green synthesis of silver nanoparticles using microbial sources has been proved to be more effective. Methods: In this study, anti-dermatophytic silver nanoparticles were synthesised by using pyrrolo metabolites producing actinomycetes as a green catalyst. Different characterization methods such as UV-Visible, XRD, and AFM were used to identify the physiochemical characteristics of synthesised nanoparticles. Results: The synthesised nanoparticles showed λ-max at 427 nm and 402 nm, respectively. The XRD analysis based on the JCPDS database identified the two synthesized nanoparticles as silver oxide nanoparticles (AgO NPs) and silver peroxide nanoparticles (Ag2O3 NPs). The size of these nanoparticles was found to be in the range of 40-44 nm (AgO NPs) and 23-25 nm (Ag2O3 NPs), respectively. The synthesized nanoparticles demonstrated significant anti-fungal activity against dermatophytic fungi Trichophyton mentagrophyes with the zone of inhibition of 38 mm by AgO NP and 17 mm by Ag2O3 NPs. Conclusion: Screening of marine actinomycetes LG003 and LG005 revealed the presence of pyrrolo derivatives as the major metabolites, suggesting that these pyrrolo derivatives could be responsible for synthesis and stabilization of AgO and Ag2O3NPs. Among the synthesized NPs, the AgO NPs showed great potential as an anti-dermatophytic agent. This study provides further research opportunities for AgONPs as anti-fungal agents.