Affiliation:
1. Department of Medical Histology and Cell Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
2. Department of Biochemistry and Molecular Biology, Faculty of Medicine, Zagazig University, Zagazig, Egypt
Abstract
Objectives:
The scientific research community devotes stupendous efforts to control
the arguable counterbalance between the undesirable effects of hormone replacement therapy
(HRT) and post-menopausal syndrome. The recent emergence of 3rd generation selective estrogen
receptor modulators and phytoestrogens has provided a promising alternative to HRT.
Hence, we assessed the potential effects of combined Bazedoxifene and Genistein on
hippocampal neuro-alterations induced by experimental ovariectomy.
Methods:
For this purpose, we utilized forty-eight healthy sexually mature female Wistar rats assorted
to control, ovariectomy (OVX), Genistein-treated ovariectomized (OVX+GEN) and
Bazedoxifene and Genistein-treated ovariectomized (OVX+BZA+GEN) groups. Hippocampi
samples from various groups were examined by H&E, silver stains and immunohistochemical
examination for calbindin-D28k, GFAP, and BAX proteins. We also assessed hippocampal
mRNA expression of ERK, CREB, BDNF and TrkB.
Results:
Our histopathological results confirmed that combined BZA+GEN induced restoration
of hippocampal neuronal architecture, significant reduction of GFAP and BAX mean area % and
significant upregulation of calbindin-D28k immunoexpression. Furthermore, we observed significant
upregulation of ERK, CREB, BDNF and TrkB mRNA expression in the BZA+GEN group
compared to the OVX group.
Conclusion:
Taken together, our findings have provided a comprehensive assessment of histological,
immunohistochemical and cyto-molecular basis of combined Genistein and Bazedoxifene
ameliorative impacts on hippocampal neuro-alterations of OVX rats via upregulation of
Calbindin, CERB, BDNF, Trk-B and ERK neuronal expression.
Publisher
Bentham Science Publishers Ltd.
Subject
General Health Professions