The Cytotoxicity Effect of Chitosan-Encapsulated Ricin-Herceptin Immunotoxin Nanoparticles on Breast Cancer Cell Lines

Abstract

Background:: The use of targeted therapy has been increasing for cancer treatment. The aim of this study is to investigate chitosan-based ricin-Herceptin (rh) immunotoxin on breast cancer cell lines. background: The targeted therapy is growing for cancer treatment. The aim here is to investigate a chitosan based Ricin-Herceptin (rh) immunotoxin on breast cancer cell lines. Methods:: The gene construct encoding immunotoxin was designed, cloned, and expressed in E. coli BL21 (DE3). The expressed proteins were isolated by the nickel-nitrilotriacetic acid column and were analyzed by the Western-blotting. The cytotoxicity of immunotoxin was assayed on breast cell line MCF-7 and using MTT assay at 24 and 48 h treatment. Results:: The immunotoxins extrication rate, size, loading percentage, and electric charge of nanoparticles were reported appropriately as 78%, 151.5 nm, 83.53%, and +11.1 mV, respectively. The encapsulated immunotoxins led to the death of 70% and 78% of MCF-7 cells at 24 and 48 h treatment, respectively. The noncapsulated counterparts at equal doses killed 53% and 62% of cancer cells at the same time points. Conclusion:: The chitosan-immunotoxins impose potential cytotoxic effects on cancer cells. other: Keywords: Herceptin, HER2, Ricin, Targeted therapy, Breast Cancer, Nanoparticles