Affiliation:
1. Department of Pharmacy, Faculdade de Farmácia, Laboratório de Controle de Qualidade, Universidade Federal de
Goiás, Goiânia-GO, Brasil
Abstract
Background:
Azithromycin (AZT), an antimicrobial, despite having a monograph in official
compendiums and some methods available in the literature, there is still a demand for eco-efficient
methods, which include green analytical chemistry.
Objective:
This study aimed to develop and validate a green spectrophotometric method for quantifying
AZT tablets.
Method:
Purified water and ethanol (90:10, v/v) and sulfuric acid 20% as a diluent and reagent, respectively,
quartz cubette and 482 nm were used. The Eco-Scale Assessment (ESA) tool was used
to evaluate the greenness of the proposed analytical method.
Results:
The proposed method was linear (20-70 μg mL-1 with a correlation coefficient of 0.9984),
precise (RSD < 5%), selective by sample adjuvants, exact (100.46%), and robust against changes
in acid proportion and wavelength. The ESA calculation was 85 points.
Conclusion:
The proposed method can be classified as an excellent green alternative to quantify
AZT in tablets, in accordance with ESA.
Publisher
Bentham Science Publishers Ltd.