Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations-Reference-Cited by-同舟云学术

Hydrophilic Interaction Liquid Chromatography for the Analysis of Pharmaceutical Formulations

Published:2024-06 Issue:5 Volume:20 Page:295-317
ISSN:1573-4110
Container-title:Current Analytical Chemistry
language:en
Short-container-title:CAC

Author:

Radoičić Aleksandra¹^ORCID,Šegan Sandra²^ORCID,Dramićanin Aleksandra³^ORCID,Milojković-Opsenica Dušanka³^ORCID

Affiliation:

1. Innovative Centre of Faculty of Chemistry Ltd. University of Belgrade, 11000, Belgrade, Serbia

2. Institute of Chemistry, Technology, and Metallurgy, University of Belgrade, Njegoševa 12, 11000, Belgrade, Serbia

3. Faculty of Chemistry, University of Belgrade, P.O. Box 51, 11158, Belgrade, Serbia

Abstract

Abstract: For a long time, Reversed-Phase Liquid Chromatography (RPLC) was the most dominant technique for the analysis of pharmaceutical compounds, but with poor efficiency in the separation of small polar molecules. From the efforts to solve the problem of insufficient retention of these molecules, during the last decades, a mode of liquid chromatography named Hydrophilic Interaction Liquid Chromatography (HILIC) has experienced vast expansion. It is based on the use of a highly hydrophilic stationary phase along with an aqueous mobile phase with high organic modifier content. In this review, the characteristics of stationary and mobile phases used in HILIC are described, and corresponding separation mechanisms are discussed. An overview of recently published papers dealing with the application of HILIC in analyzing pharmaceuticals in biological and non-biological samples is provided. Besides, the application of HILIC systems in the determination of the physicochemical properties of compounds is described.

Funder

Ministry of Education, Science and Technological Development of the Republic of Serbia

Publisher

Bentham Science Publishers Ltd.

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