Affiliation:
1. ICAR-Indian Institute of Pulses Research, Kanpur, 208024, India
2. ICAR-Indian Agricultural Statistics Research Institute, New Delhi- 110012, India
Abstract
Background:
Chickpea is a nutritional rich premier pulse crop but its production encounters
setbacks due to various stresses and understanding of molecular mechanisms can be ascribed foremost importance.
Objective:
The investigation was carried out to identify the differentially expressed WRKY TFs in chickpea
in response to herbicide stress and decipher their interacting partners.
Methods:
For this purpose, transcriptome wide identification of WRKY TFs in chickpea was done. Behavior
of the differentially expressed TFs was compared between other stress conditions. Orthology based cofunctional
gene networks were derived from Arabidopsis. Gene ontology and functional enrichment analysis
was performed using Blast2GO and STRING software. Gene Coexpression Network (GCN) was constructed
in chickpea using publicly available transcriptome data. Expression pattern of the identified gene
network was studied in chickpea-Fusarium interactions.
Results:
A unique WRKY TF (Ca_08086) was found to be significantly (q value = 0.02) upregulated not
only under herbicide stress but also in other stresses. Co-functional network of 14 genes, namely
Ca_08086, Ca_19657, Ca_01317, Ca_20172, Ca_12226, Ca_15326, Ca_04218, Ca_07256, Ca_14620,
Ca_12474, Ca_11595, Ca_15291, Ca_11762 and Ca_03543 were identified. GCN revealed 95 hub genes
based on the significant probability scores. Functional annotation indicated role in callose deposition and
response to chitin. Interestingly, contrasting expression pattern of the 14 network genes was observed in
wilt resistant and susceptible chickpea genotypes, infected with Fusarium.
Conclusion:
This is the first report of identification of a multi-stress responsive WRKY TF and its associated
GCN in chickpea.
Funder
Indian Council of Agricultural Research (ICAR)-Indian Institute of Pulses Research (IIPR), Kanpur, India
Publisher
Bentham Science Publishers Ltd.
Subject
Computational Mathematics,Genetics,Molecular Biology,Biochemistry
Cited by
5 articles.
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