Enhanced Release Kinetics and Stability of Resveratrol Loaded Self Nanoemulsifying Delivery Systems Developed using Experimental Design

Abstract

Background: Resveratrol is a member of the stilbene family emerged as a leading candidate for improving healthspan through potentially slowing the aging process and preventing chronic diseases. A number of institutions and scientists specialized in this field across the world are working to develop a promising Self Emulsifying formulation to enhance bioavailability of hydrophobic resveratrol using oil. Objective: The objective of the current study is to develop self-nano emulsifying drug delivery systems using long-chain triglycerides of resveratrol to enhance solubility, stability, release kinetics and to overcome low bioavailability. Methods: Solubility studies performed in different lipids, surfactants and cosurfactants. Phase diagrams constructed to select the areas of nanoemulsion. SNEDDS formulation was optimized using 33 central composite design considering lipid (X1), surfactant (X2) and co-surfactant (X3) as critical variables, optimized formulation was located using overlay plot. Results: The nanometer size and high values of zeta potential depicted non-coalescent nature of SNEDDS. The resulted SNEDDS formulation had improved in vitro release followed by Hixson Crowell model with higher regression R2value 0. 929. Thermodynamic stability studies ascertained stable formulation. Mean droplet size in selected nanocarrier was found to be 83.29 nm. The nanocarriers subjected to 2-8°C (45% RH), 25-30°C (60% RH) and 45-50°C (75% RH) in glass vials exhibited no significant changes in 3 months. Conclusion: The novel approach was developed by selecting optimum blends of lipids, surfactants and cosurfactant using central composite design. This study not only offers a good example of augmenting bioavailability of resveratrol but will also provide a promising oral formulation for clinical application.