Affiliation:
1. Department of Biology, Faculty of Biology, Alexandru Ioan Cuza University of Iasi,Romania
2. Center for Fundamental Research and Experimental Development in Translation Medicine-TRANSCEND, Regional Institute of Oncology, Iasi,Romania
Abstract
Background:
The conifer species Pinus halepensis (Pinaceae) and Tetraclinis articulata
(Cupressaceae) are widely used in traditional medicine due to their beneficial health properties.
Objective:
This study aimed to investigate the mechanisms by which P. halepensis and T. articulata
essential oils (1% and 3%) could exhibit neuroprotective effects in an Alzheimer's disease
(AD) rat model, induced by intracerebroventricular (i.c.v.) administration of amyloid beta1-42
(Aβ1-42).
Method:
The essential oils were administered by inhalation to the AD rat model, once daily, for 21
days. DNA fragmentation was assessed through a Cell Death Detection ELISA kit. Brain-derived
neurotrophic factor (BDNF), activity-regulated cytoskeleton-associated protein (ARC), and interleukin-
1β (IL-1β) gene expressions were determined by RT-qPCR analysis, while BDNF and ARC
protein expressions were assessed using immunohistochemistry technique.
Results:
Our data showed that both essential oils substantially attenuated memory impairments,
with P. halepensis mainly stimulating ARC expression and T. articulata mostly enhancing BDNF
expression. Also, the inhalation of essential oils reduced IL-1β expression and induced positive effects
against DNA fragmentation associated with Aβ1-42-induced toxicity, further contributing to
the cognitive improvement in the rats with the AD-like model.
Conclusion:
Our findings provide further evidence that these essential oils and their chemical constituents
could be natural agents of therapeutic interest against Aβ1-42-induced neurotoxicity.
Publisher
Bentham Science Publishers Ltd.
Subject
Pharmacology,General Neuroscience
Cited by
3 articles.
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