Protocols for the Detection and Proteome Analysis of the Yellow Mosaic Virus Infected Soyabean Leaves

Abstract

Soybean (Glycine max) is one of the legumes, susceptible to yellow mosaic disease caused by Mungbean yellow mosaic India virus (MYMIV) and Mungbean yellow mosaic virus (MYMV) infection. The quantitative proteomic analysis allows achieving deeper knowledge about the viral infection. For quantitative proteomic analysis, two-dimensional gel electrophoresis (2D-PAGE) is the common method of choice. Optimization is required even for the published protocols based on the type of sample to be analyzed and for the proteins of interest. We compared four different published protocols with some modifications and selected the one which is more effective in terms of resolution and reproducibility of 2D-PAGE. Here we present our simple and cost-effective procedure for the detection of viral infection and proteomic analysis of YMV infected soybean leaves without compromising the resolution and reproducibility of 2D-PAGE.