Abstract
Genomic DNA extraction is the starting point for various downstream
molecular biology applications viz. PCR, restriction analysis, hybridisation etc.
Numerous problems like DNA degradation, co-isolation of viscous polysaccharides,
polyphenols and other secondary metabolites causing damage to DNA, inhibiting
restriction enzymes, DNA polymerases etc, are routinely encountered during DNA
isolation from plants. Quinone compounds resulting from oxidation of polyphenols
lead brown the DNA preparations and can also damage proteins and DNA’s due to
their oxidizing properties. This results in a poor yield of high molecular weight DNA.
The protocol below explains the extraction of DNA via the CTAB method, involving
three major steps viz lysis of cell wall and membranes, extraction of genomic DNA and
precipitation of DNA.
Publisher
BENTHAM SCIENCE PUBLISHERS