Affiliation:
1. Department of Pharmaceutical Analysis, ISF College of Pharmacy Moga, 142001, Punjab, India
Abstract
Abstract:
In recent years, size exclusion chromatography (SEC) has gained valuable and impactable
recognition among various chromatographic techniques. Also addressed as other names, viz. gel permeation
chromatography, steric-exclusion chromatography, etc., SEC is typically taken into consideration for
the fractionation and molecular weight determination of biomolecules and large macromolecules (proteins
and polymers) using porous particles. A homogenous mixture of molecules dispersed in the mobile phase
is introduced to the chromatographic column, which provides a solid support in the form of microscopic
beads (the stationary phase). The beads act as “sieves” and purify small molecules, which become temporarily
trapped inside the pores. Some of the advantages that SEC offers over other chromatographic techniques
are short analysis time, no sample loss, good sensitivity, and requirement for less amount of mobile
phase. In the proposed manuscript, we have deliberated various proteomic applications of size exclusion
chromatography, which include the isolation of extracellular vesicles in cancer, isolation of human
synovial fluid, separation of monoclonal antibodies, as well as several tandem techniques, such as deep
glycoproteomic analysis using SEC-LC-MS/MS, analysis of mammalian polysomes in cells and tissues
using tandem MS-SEC, SEC-SWATH-MS profiling of the proteome with a focus on complexity, etc.
Publisher
Bentham Science Publishers Ltd.