Affiliation:
1. Active Pharmaceutical Ingredients Research Center, Pharmaceutical Sciences Research Center, Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, Iran
Abstract
Introduction:
Verapamil (Verap) is an antidysrhythmic agent and a calcium channel blocker,
indicated for angina, hypertension, supraventricular arrhythmias, and migraine.
Objective:
Drug monitoring plays a critical role in patient survival. In order to prevent the onset of
drug toxicity, trace levels of this drug should be determined.
Methods:
For this reason, solvent bar microextraction technique coupled with high-performance liquid
chromatography was implemented.
Results:
Under optimum condition, verapamil was micro-extracted from a donor solution (pH=11) to
an acceptor solution (pH=3.2). It was transferred through n-octanol as the organic solvent, which was
impregnated in the pores of the hollow fiber. Salt addition (30%) had the major effect on the efficiency
of the method. Interaction of time (65 min), temperature (25°C), and stirring rate (818 rpm) had a
significant effect too. It all resulted in a limit of detection and quantification of 15 ng mL-1 and 50 ng mL-1,
respectively. The relative standard deviations of analysis were 4.9% within a day (n=3) and 5.7%
between days (n=9). The calibration curves represented good linearity for urine and plasma samples
with coefficient estimations higher than 0.99 with a linearity range of 50-5000 ng mL-1. The relative
standard deviation for intra- (n=3) and inter-(n=9) day was 4.2% and 5.7%, respectively.
Conclusion:
It could be concluded that the application of this method for dose monitoring can be done
at hospital and healthcare facilities.
Publisher
Bentham Science Publishers Ltd.
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献