Affiliation:
1. School of Chemical and Biotechnology, SASTRA Deemed University, Tirumalaisamudram,
Thanjavur 613401, Tamil Nadu, India
2. Laboratory for Venom Peptidomics and Molecular Simulations, Department of Biotechnology, Bannari Amman Institute
of Technology, Erode 638401, Tamil Nadu, India
3. Department of Biotechnology, Saveetha School of Engineering,
Thandalam, Tamilnadu 600100, India
Abstract
Background:
Psoralea corylifolia and Mimusops elengi could be a source of new bioactive
compounds for treating filariasis Psoralea corylifolia and Mimusops elengi. Thediversity of these
plants has been proved medicinally and phytochemically. These plants haveplayed an important role in
ancient medicine for the treatments of various ailments and especially against many helminths.
Methods:
Psoralea corylifolia and Mimusops elengi could be a source of new bioactive compounds
for treating filariasis. 20g of dried powder of Psoralea corylifolia and Mimusops elengi were extracted
using a Soxhlet apparatus using various solvents like methanol, chloroform, acetone, and
water for about 30 cycles and then concentrated using a rotary evaporator and stored at 4 °C in airtight
containers. It is effective against Setaria cervi at 100 and 200 μg, respectively. In-vitro studies
have been done for the respective compounds. Homology models of Glutathione S transferase, Chitinase,
and Transglutaminase were constructed using MODELLER v9.20 (Accelrys).
Results:
The results from the in vitro studies were confirmed by TLC and GC MS analysis of the
extracts, which showed the presence of different phytochemical compounds in the extracts. Among
the compounds identified in GC MS, 7,10-Octadecadienoic acid, methyl ester, Pentadecanoic acid,
14-methyl-, methyl ester, 2-Nonadecanone2,4-dinitrophenylhydrazine, Aspidospermidin-17-ol, 1-
acetyl-19,21-epoxy-15,16-dimethoxy-, 9- (2’,2’-Dimethylpropanihydrozano)-3,6 dichloro-2,7-bis-
[2-(diethylamino)-ethoxy] fluorine, and 2,2,4-Trimethyl-3-(3,8,12,16-tetramethyl-heptadeca-
3,7,11,15-tetraenyl)-cyclohexanol were docked against crystallised bioactive compounds.
Conclusion:
As a result of this study, it was concluded that arresting these proteins will help in arresting
the growth and multiplication of parasites, thus leading to the development of a new antifilarial
drug.
Publisher
Bentham Science Publishers Ltd.