Isolation, Screening and Antimicrobial Activity of Aeromonas hydrophila from Spoiled Vegetables and Biochemical Analysis of Estuarine Microbial Sediments

Abstract

Background: Aeromonas hydrophila is a heterotrophic, Gram-negative, rod-shaped, facultative anaerobic, non-spore forming bacteria that are autochthonous and widely dispersed in marine environments. The study aims at investigating the screening of Aeromonas hydrophila from spoiled vegetables and the sediment sample collected from three different estuaries located in the Bay of Bengal (Vedharanyam, Parangipettai and Pichavaram, Tamilnadu, India) for the presence of enzymes and antimicrobial activities. Objective: Isolation, enzyme screening, antimicrobial activity of Aeromonas hydrophila from spoiled vegetables and three different estuarine microbial sediment samples for the purpose of biochemical and enzymatic analysis. Methods: The bioactive compound produced by this strain was purified by using thin-layer chromatography. Results: The purified isolate of Aeromonas hydrophila strain produces good antimicrobial activity against Aspergillus niger, Candida albicans, Staphylococcus, Klebsiella and pseudomonas species. Conclusion: These isolates producing amylase, protease, lipase, and gelatinase enzymes, which are commercially very important and used in many industries and other biochemical sectors.