Affiliation:
1. Plastic Surgery, The First Affiliated Hospital of Wenzhou Medical University, Nanbaixiang, Wenzhou City, Zhejiang Province,
325000, China
2. Department of Cosmetology, Wenzhou Hospital of Integrated Traditional Chinese and Western Medicine, Wenzhou,
Zhejiang, 325000, China
3. Wenzhou Key Laboratory of Laser Cosmetology, Wenzhou Hospital of Integrated Traditional Chinese and
Western Medicine, Wenzhou, Zhejiang, 325000, China
Abstract
Aim:
This study aims to explore the potential of Osmundacetone (OSC) as a new treatment for infantile
hemangiomas (IH), the most common benign tumors in infancy. Currently, propranolol serves as the primary
treatment for IH, but its effectiveness is limited, and it poses challenges of drug resistance and side effects. Therefore,
there is a pressing need to identify alternative therapies for IH.
Methods:
The effects of OSC on the proliferation and apoptosis of HemECs (endothelial cells from hemangiomas)
were assessed using CCK-8 assay, colony formation assay, HOCHEST 33342 staining, and flow cytometry.
Western blot analysis was performed to investigate OSC's influence on Caspases and angiogenesis-related proteins.
Animal models were established using HemECs and BALB/c mice, and histological and immunohistochemical
staining were conducted to evaluate the impact of OSC on mouse hemangiomas, VEGFR2, and MMP9
expression.
Results:
OSC treatment significantly reduced HemECs' viability and colony-forming ability, while promoting
apoptosis, as indicated by increased HOCHEST 33342 staining. OSC upregulated the protein expression of Bax,
PARP, Caspase9, Caspase3, AIF, Cyto C, FADD, and Caspase8 in HemECs. In animal models, OSC treatment
effectively reduced hemangioma size and improved histopathological changes. OSC also suppressed VEGFR2
and MMP9 expression while elevating Caspase3 levels in mouse hemangiomas.
Conclusion:
OSC demonstrated promising results in inhibiting HemECs' proliferation, inducing apoptosis, and
ameliorating pathological changes in hemangiomas in mice. Moreover, it influenced the expression of crucial
caspases and angiogenesis-related proteins. These findings suggest that OSC holds potential as a novel drug for
clinical treatment of IH.
Publisher
Bentham Science Publishers Ltd.
Subject
Cancer Research,Pharmacology,Molecular Medicine