The Cell Biocompatibility of a new Polycaprolactone-based Endodontics Sealer Compared with AH Plus Sealer

Abstract

Background:One of the important materials needed for root treatment is the sealers. They fill the space between the gutta and the canal walls and create a suitable seal to prevent the colonization of oral microorganisms in the peri-apical tissues and inside the canal space.Aim:The aim of this study was to prepare and test the cell biocompatibility of a new polycaprolactone-based endodontics sealer in comparison to AH plus sealer against dental pulp stem cells.Methods:MTT assay was used to evaluate the cytotoxicity.Results:The results showed that AH plus showed moderate cytotoxicity effects against cells (the number of living cells was 49.45 ± 10.56%). However, for the new sealer, the number of living cells was ≥ 80% (81% ± 4.11). Thus, the sample was non-cytotoxic against tested cells.Conclusion:More investigations are needed on the new sealer to validate its biocompatibility and usefulness.