GC-MS/MS Analysis and Wound Repair Potential of Urtica dioica Essential Oil: In silico Modeling and In vivo Study in Rats-Reference-Cited by-同舟云学术

GC-MS/MS Analysis and Wound Repair Potential of Urtica dioica Essential Oil: In silico Modeling and In vivo Study in Rats

Published:2024-07-05 Issue: Volume:25 Page:
ISSN:1389-2010
Container-title:Current Pharmaceutical Biotechnology
language:en
Short-container-title:CPB

Author:

Chira Ahlem¹,Kadmi Yassine²³,Badraoui Riadh⁴⁵,Aouadi Kaïss⁶⁷,Alhawday Fahad⁶,Boudaya Mariem⁸,Jamoussi Kamel⁸,Kallel Choumous⁹,El Feki Abdelfattah¹,Kadri Adel¹⁰¹¹,Saoudi Mongi¹

Affiliation:

1. Laboratory of Biomathematics LR22ES01, Faculty of Sciences of Sfax, BP 1171 Sfax 3000, Tunisia

2. LASIRE CNRS UMR 8516, Université Lille, Sciences et Technologies, Villeneuve d’Ascq, Cedex 59655, France

3. Université d’Artois, IUT de Béthune, 62400, Béthune, France

4. Laboratory of General Biology, University of Ha'il, Ha'il 45851, Saudi Arabia

5. Section of Histology-Cytology, Medicine Faculty of Tunis, University of Tunis El Manar, 1007 Tunis-La Rabta, Tunisia

6. Department of Chemistry, College of Science, Qassim University, Buraidah 51452, Saudi Arabia

7. Department of Chemistry, Laboratory of Heterocyclic Chemistry Natural Product and Reactivity/CHPNR, Faculty of Science of Monastir, University of Monastir, Avenue of the Environment, Monastir 5019, Tunisia

8. Biochemistry Laboratory, CHU Hedi Chaker of Sfax, Tunisia

9. Haematology Laboratory, Hospital Habib Bourguiba, Sfax, Tunisia

10. Faculty of Science and Arts in Baljurashi, Al-Baha University, P.O. Box (1988). Al-Baha 65527, Saudi Arabia

11. Faculty of Science of Sfax, Department of Chemistry, University of Sfax, B.P. 1171, 3000 Sfax, Tunisia

Abstract

Background: The study aimed to assess the antioxidant and wound healing properties of Urtica dioica essential oil (UDEO) through a comprehensive evaluation involving in silico, in vitro, and in vivo analyses. The phytochemistry of UDEO was also investigated to identify trace compounds crucial. Methods: Various injection methods of the multimode inlet (MMI) in chromatography were investigated to attain lower instrumental detection limits. Subsequently, in silico studies were employed to delve deeper into the potential biological activities of the identified compounds. Standard antioxidative tests, encompassing ABTS•+ and TAC, were performed. In vivo tests centered on wound healing were implemented using rat models. The rats were randomly allocated to four groups: saline solution, vaseline vehicle, cytol centella, and 5% UDEO ointment. Wound healing progress was evaluated through a chromatic study. Results: Gas chromatography combined with triple quadrupole mass spectrometry (GC-MS/MS) analysis revealed the presence of 97 thermolabile compounds in UDEO. Subsequent in silico studies unveiled the potential of identified compounds to inhibit COX-2, TNF-α, and IL-6, suggesting a possible enhancement of anti-inflammatory responses and healing processes. In vitro tests elucidated the notable antioxidant capacity of UDEO, a finding reinforced by wound healing data, revealing a substantial closure rate of 89% following the topical application of UDEO. Notably, fibrinogen and C-reactive protein (CRP) levels were significantly reduced, indicating minimized oxidative stress damage compared to control. Additionally, UDEO exhibited an increase in antioxidant enzyme activities compared to control. Conclusion: The study concludes that UDEO possesses significant antioxidant and wound-healing properties, supported by its rich phytochemical composition. The findings suggest its potential application in therapeutic interventions for oxidative stress and inflammatory conditions.

Publisher

Bentham Science Publishers Ltd.