Standardized Criterion of Presepsin Reference Interval for Accurate and Early Diagnosis of Sepsis

Abstract

Background: Presepsin is a soluble analog of CD14, recently validated as a new biomarker for sepsis. Different cut-off levels of Presepsin for the diagnosis of sepsis have been proposed however, the optimum threshold for early clinical discrimination of sepsis from non-infectious conditions remains unknown. Objective: This work was undertaken to define the reference interval of Presepsin for Sepsis according to the CLSI C28-A3c approved guideline. Methods: Reference individuals (N=72) receiving in-patient care at ICU for sepsis and complications were selected for the study. Presepsin concentrations were measured by enzyme-linked immunosorbent assay. Reference limits were calculated using the non-parametric robust method. Results: Overall, the reference range for Presepsin in sepsis patients was 24.2 -872.2 pg/mL (90% confidence interval). Peak values for males were relatively higher than for females and the presepsin concentrations were influenced by age. Conclusion: Proposed reference interval could assist in accurate and early diagnosis of sepsis which would eventually improve the outcome in patients of all age groups.