Differentiation of Pluripotent Stem Cells into Nucleus Pulposus Progenitor Cells for Intervertebral Disc Regeneration
-
Published:2019-01-14
Issue:1
Volume:14
Page:57-64
-
ISSN:1574-888X
-
Container-title:Current Stem Cell Research & Therapy
-
language:en
-
Short-container-title:CSCR
Author:
Xia Kaishun1, Gong Zhe1, Zhu Jian1, Yu Wei1, Wang Yitian1, Wang Junjie1, Xu Ankai1, Zhou Xiaopeng1, Tao Huimin1, Li Fangcai1, Liang Chengzhen1
Affiliation:
1. Department of Orthopedics, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, No. 88 Jie Fang Road, Hangzhou, 310009, Zhejiang, China
Abstract
Low back pain (LBP) is one of the world’s most common musculoskeletal diseases and is frequently associated with intervertebral disc degeneration (IDD). While the main cause of IDD is commonly attributed to a reduced number of nucleus pulposus (NP) cells, current treatment strategies (both surgical and more conservative) fail to replenish NP cells or reverse the pathology. Cell replacement therapies are an attractive alternative for treating IDD. However, injecting intervertebral disc (IVD) cells, chondrocytes, or mesenchymal stem cells into various animal models of IDD indicate that transplanted cells generally fail to survive and engraft into the avascular IVD niche. Whereas pluripotent stem cells (PSCs), including induced pluripotent stem cells (iPSCs) and embryonic stem cells (ESCs), hold great potential for revolutionizing regenerative medicine, current protocols for differentiating these cells into NP-like cells are inadequate. Nucleus pulposus progenitor cells (NPPCs), which are derived from the embryonic notochord, can not only survive within the harsh hypoxic environment of the IVD, but they also efficiently differentiate into NP-like cells. Here we provide an overview of the latest progress in repairing degenerated IVDs using PSCs and NPPCs. We also discuss the molecular pathways by which PSCs differentiate into NPPCs in vitro and in vivo and propose a new, in vivo IDD therapy.
Funder
Leona M. and Harry B. Helmsley Charitable Trust National Natural Science Foundation of China Health foundation of Zhejiang Province Science and Technology Foundation of Zhejiang Province
Publisher
Bentham Science Publishers Ltd.
Subject
General Medicine,Medicine (miscellaneous)
Reference83 articles.
1. Vos T, Flaxman AD, Naghavi M. Lancet, Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010.,, 2012, 380,, 2163-2196, 2. Henriksson HB, Brisby H. Cells Tissues Organs, Development and regeneration potential of the mammalian intervertebral disc.,, 2013, 197,, 1-13, 3. Ricci JA, Stewart WF, Chee E. Spine (Phila Pa 1976), Back pain exacerbations and lost productive time costs in United States workers.,, 2006, 31,, 3052-3060, 4. Martin BI, Deyo RA, Mirza SK. JAMA, Expenditures and health status among adults with back and neck problems.,, 2008, 299,, 656-664, 5. Pettine KA, Murphy MB, Suzuki RK, Sand TT. Stem Cells, Percutaneous injection of autologous bone marrow concentrate cells significantly reduces lumbar discogenic pain through 12 months.,, 2015, 33,, 146-156,
Cited by
20 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献
|
|