Affiliation:
1. Dental Research Center, Research Institute of Dental Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2. Department of Periodontics, Dental School of Shahid Beheshti University of Medical Sciences, Tehran, Iran
Abstract
Abstract:
Despite the plethora of literature regarding isolation and characterization of periodontal ligament
stem cells (PDLSCs), due to the existence of controversies in the results, in this comprehensive
review, we aimed to summarize and compare the effect of isolation methods on PDLSC properties, including
clonogenicity, viability/proliferation, markers expression, cell morphology, differentiation, and
regeneration. Moreover, the outcomes of included studies, considering various parameters, such as teeth
developmental stages, donor age, periodontal ligament health status, and part of the teeth root from which
PDLSCs were derived, have been systematically discussed. It has been shown that from included studies,
PDLSCs can be isolated from teeth at any developmental stages, health status condition, and donor age.
Furthermore, a non-enzymatic digestion method, named as an explant or outgrowth technique, is a suitable
protocol for PDLSCs isolation.
Publisher
Bentham Science Publishers Ltd.
Subject
General Medicine,Medicine (miscellaneous)
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