Affiliation:
1. Sports Medicine Department, Northern
Jiangsu People’s Hospital, Clinical Medical College, Yangzhou University, Yangzhou 225001, People’s Republic of
China
2. Dalian Medical University, Dalian 116044, People’s Republic of China
Abstract
Background:
Acute skeletal muscle injuries are common physical or sports traumas. Cellular
therapy has excellent potential for regeneration after skeletal muscle injury. Adipose-derived stem cells
(ADSCs) are a more accessible type of stem cell. However, it has a low survival rate and differentiation
efficiency in the oxidative stress-rich microenvironment after transplantation. Although molecular hydrogen
(H2) possesses anti-inflammatory and antioxidant biological properties, its utility in mitochondrial
and stem cell research has not been adequately explored.
Objective:
This study aimed to reveal the role of H2 on adipose-derived stem cells' myogenic differentiation.
Methods:
The protective effects of H2 in ADSCs were evaluated by MTT assay, live-dead cell staining,
western blot analysis, immunofluorescence staining, confocal imaging, and transmission electron microscopy.
Results:
An appropriate volume fraction of H2 significantly decreased mitochondrial reactive oxygen
species (ROS) levels, increased the number of mitochondria, and promoted mitophagy, thus enhancing
the survival and myogenic differentiation of ADSCs.
Conclusion:
This study reveals the application potential of H2 in skeletal muscle diseases or other pathologies
related to mitochondrial dysfunction.
Publisher
Bentham Science Publishers Ltd.
Subject
General Medicine,Medicine (miscellaneous)
Cited by
5 articles.
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