Using a 3-Steps Supercritical Fluids Assisted Process for the Generation of Nanostructured Biopolymeric Scaffolds

Author:

Cardea Stefano1

Affiliation:

1. Department of Industrial Engineering, University of Salerno, Via Giovanni Paolo II, 84084, Fisciano, Italy

Abstract

Background: Scaffolds can be used to substitute the extracellular matrix and to favour the generation of tissues and organs. Until now, various processes have been implemented for scaffolds generation, but they are characterized by several limits. Methods: In this work, we tested a supercritical fluids assisted process for the generation of nano-structured biopolymeric scaffolds; it is characterized by three steps: generation of a polymeric gel (loaded with a porogen), drying of the gel using supercritical CO2, waterwashing to remove the porogen. Results: 3D Poly(D,L-lactic acid) scaffolds have been obtained, characterized by very high porosity (> 90%) and surface are (> 200 m2/g), and by a fibrous nanostructure (fibres ranging between 60 and 400 nm) superimposed to a micrometric cellular structure. Conclusion: Moreover, suitable mechanical properties (up to 125 KPa) and very low solvents residue (< 5 ppm) have been obtained.

Publisher

Bentham Science Publishers Ltd.

Subject

General Chemical Engineering

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