3,6-dichloro-1,2,4,5-Tetrazine Assayed at High Doses in the Metastatic Breast Cancer Cell Line MDA-MB-231 Reduces Cell Numbers and Induces Apoptosis

Author:

Abdelkarim Mohamed1,Ben Younes Khaoula1,Limam Ines1,Guermazi Refka2,ElGaaied Amel Ben Ammaar3,Aissa-Fennira Fatma Ben1

Affiliation:

1. Laboratory of onco-hematology, Faculty of medicine of Tunis, Tunis El Manar University, Tunis, Tunisia

2. Laboratory of Organic Molecular Chemistry, ENSIT MONTFLEURY 5 Taha Hussein Avenue 1008 Montfleury, University of Tunis, Tunis, Tunisia

3. Laboratory of Genetics Immunology and Humans Pathologies, Faculty of sciences of Tunis, Tunis El Manar University, Tunisia

Abstract

Background: The lack of treatment options for patients with chemotherapy-resistant cancers is pushing the field toward the development of new therapies. 1,2,4,5-tetrazine derivatives are a class of heterocyclic compounds that exhibit a broad spectrum of antitumor activities. Objective: The purpose of this study was to assess the biological activity of four s-tetrazine derivatives by substitution of two chloride atom of 3,6-dichloro-1,2,4,5-tetrazine with long hydrophobic side chains. Methods: We analyzed the anti-proliferative effects of four s-tetrazine derivatives with MTT assay and their pro-apoptotic effect with AV/ IP flow cytometry analysis and Hoechst 33342 staining. Results: We demonstrated that 3,6-dichloro-1,2,4,5-tetrazine (compound (1)) has a cytotoxic effect and induces apoptosis. Conclusion: 3,6-dichloro-1,2,4,5-tetrazine presents a new cytotoxic drug against metastatic breast cancer cell line MDA-MB-231 in vitro.

Publisher

Bentham Science Publishers Ltd.

Subject

General Pharmacology, Toxicology and Pharmaceutics

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