Marine Streptomyces sp. VITASP as a Source of New Bioactive Secondary Metabolites

Abstract

Background:: The main aim of the study was to evaluate the bioactive properties of ethyl acetate crude extract of Streptomyces sp. VITASP with a view to assess their therapeutic potential. Methods: The morphological, physiological and the biochemical properties of the strain Streptomyces sp. VITASP were confirmed by conventional methods. The present study evaluated the antibacterial, antioxidant and cytotoxic activities. Results: The isolate was identified to be Streptomyces sp. (Genbank accession number: KR233807). The ethyl acetate extract of Streptomyces sp. VITASP showed maximum antibacterial activity of two Gram- positive and Gram- negative bacteria at 0.5mg/mL. The antioxidant potential of the crude extract exhibited strong reducing power activity at 0.5 mg/mL with 93±0.05% inhibition. The antiinflammatory and anti-diabetic properties were identified at 0.5 mg/mL concentration. The cytotoxic effect was found with an IC50 of 500μg/ mL on HeLa cell lines. The GC-MS analysis and the chromatogram patterns revealed major peak at 18.485 which corresponds to Pyrrolo[1,2-a]pyrazine-1,4-dione, Hexahydro-3-(2-methylpropyl. IR spectra showed the functional groups. HPLC Retention time of the peak was found to be 2.414 min. Conclusion: This work demonstrates that the extract should be considered as a useful source as an antioxidant and antimicrobial agent and gives further insight into the potential use of the compounds as drugs for various other bioactivities.