Affiliation:
1. Adarsh Vijendra Institute of Pharmaceutical Sciences, Shobhit University, Gangoh, Uttar Pradesh - 247341, India
2. Goel Institute of Pharmacy & Sciences Lucknow, Uttar Pradesh-226028, India
Abstract
Background:
The main aim of the study was to compare the neuroprotective potential
of Polyherbal Formulation (PHF) with that of an extract of a well-reported anti-parkinson plant,
i.e., Mucuna pruriens.
Methods:
Different PHF combinations (PHFs) were formulated by using hydro-alcoholic extracts
and were tested for neuroprotective potential against Mucuna pruriens extract (MPE). In the experimental
study, 30 albino mice (Swiss strain, 35-45g) were grouped into Control, MPTP, MPTP+
MPE, MPTP+PHFs, MPTP+ L-DOPA groups. Experimental mice were given PHFs and MPE
(50 mg/kg body wt.) by intraperitoneal routes. MPTP (1-methyl 4-phenyl 1,2,3,6-tetrahydropyridine)
was given orally for 2 weeks with prior use of PHFs and MPE 20 mg/kg body wt. for 2
weeks. After treatment, a neurobehavioral study was performed as well as neurochemical parameters
were evaluated.
Results:
The results showed that polyherbal formulation improved the performance of the dopaminergic
neurons in the substantia nigra region of the brain compared to MPE with respect to MPTP intoxication.
A significant reduction was found in spontaneous locomotor activity and rotarod activity
in MPTP treated mice in contrast with the control group, in whom these activities were restored
by MPTP+MPE and MPTP+PHF1; however, this contrasted with the standard L-Dopa treatment
group. This improvement was observed to be significantly better in the MPTP+PHF1 treated group
compared to the treatment group of MPTP+MPE. The changes in different parameters occurred after
the MPTP treatment. These changes were observed in the levels of malondialdehyde (MDA),
conjugated dienes (CD), superoxide dismutase (SOD), and catalase.
Conclusion:
The study concluded that PHF treatment promotes significant neurogenesis, reduces
apoptosis, promotes antioxidant capacity, and restores dopamine levels. PHF contains numerous
classes of chemical constituents, which show a synergistic effect for better therapeutic remuneration
and neuroprotection compared to the single chemical entity L-DOPA, which is a well-known
chemical constituent present in MPE.
Publisher
Bentham Science Publishers Ltd.
Subject
General Pharmacology, Toxicology and Pharmaceutics
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